金英黄归汤对LPS介导乳腺上皮细胞TLR4信号通路中相关因子的影响

doi:10.11843/j.issn.0366-6964.2016.03.026

Abstract

Abstract:

The study was conducted to investigate the effects of Jin-Ying-Tang （JYT） on correlated molecules of TLR4NF-κB signaling of LPS-stimulated mouse mammary epithelial cells(MECs) in vitro and explore its underlying molecular mechanisms.ELISA was performed to detect changes of the content of IL-8 and TNF-α in the culture supernatants.The mRNA transcription of TLR4-NF-κB signaling molecules such as IL-1 receptor-associated kinase-1(IRAK-1)，tumour necrosis factor receptor associated factor 6(TRAF-6)，Transforming Growth Factor activated kinase 1(TAK-1)，NF-κB inducing kinase(NIK)，Inhibitor of κB(IκB) were determined by qRT-PCR.The results showed that JYT could significantly decrease the expression of IL-8 and TNF-α in LPS-stimulated MECs(P＜0.05，P＜0.01)；what’s more，JYT could down-regulate the expression of IRAK-1，TRAF-6，TAK-1，IκB，NIK mRNA activated by LPS(P＜0.05，P＜0.01).It is concluded that JYT attenuates LPS-induced inflammatory response in MECs by inhibiting the activitation of IRAK-1/TRAF-6/TAK-1/NIK pathway，but not of IRAK-1/TRAF-6/TAK-1/IκB pathway.

CLC Number:

S853.9

LI Xin，LI Zhong-gai，ZHANG Xiao-yi，ZHOU Song-gui，WANG Yu-kun，FENG Jiang，YI Qiong，WANG Lu. Effects of Jin-Ying-Tang on Correlated Molecules of Toll-like Receptor 4 Signaling of LPS-Induced Mouse Mammary Epithelial Cells in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 609-614.

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Effects of Jin-Ying-Tang on Correlated Molecules of Toll-like Receptor 4 Signaling of LPS-Induced Mouse Mammary Epithelial Cells in vitro

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